Background:

mOrange is extremely bright orange fluorescent protein monomers which can be used as tags or reporters. mOrange fluorescent proteins is mutants derived from mRFP1, a monomeric mutant of DsRed, which was developed in Dr. Roger Tsien’s lab by directed mutagenesis. mOrange excitation and emission maxima are 548 and 562 nm, respectively.

Applications:

WB, IHC

Name of antibody:

mOrange

Immunogen:

mOrange tagged recombinant protein

IHC positive control:

Frozen section Brain tissue

IHC Recommend dilution:

25-100

WB Positive control:

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mOrange ne) cDNA for 48 hrs and lysed

WB Recommended dilution:

500-2000