Background:

The far-red fluorescent protein mKate (lambda(ex), 588 nm; lambda(em), 635 nm; chromophore-forming triad Met(63)-Tyr(64)-Gly(65)), originating from wild-type red fluorescent progenitor eqFP578 (sea anemone Entacmaea quadricolor), is monomeric and characterized by the pronounced pH dependence of fluorescence, relatively high brightness, and high photostability. The protein has been crystallized at a pH ranging from 2 to 9 in three space groups, and four structures have been determined by x-ray crystallography at the resolution of 1.75-2.6 A.

Applications:

WB

Name of antibody:

mKate

Immunogen:

This antibody is raised against a recombinant protein and recognizes mKate.

Full name:

mKate

WB Predicted band size:

27 kDa

WB Positive control:

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mKate ne) cDNA for 48 hrs and lysed

WB Recommended dilution:

500-2000