Background:

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.

Applications:

WB, IF, IP

Name of antibody:

tGFP

Immunogen:

Purified tGFP protein expressed in HEK293T cell

SwissProt:

P42212

WB Predicted band size:

26 kDa

WB Positive control:

randomly picked 7 different tGFP tagged over-expression lysates

WB Recommended dilution:

1000-5000

IF Positive control:

Cos7 cells transiently transfected with turboGFP tagged LAMP1 ORF cDNA clone

IF Recommended dilution:

100-200

IP Recommend dilution:

50-200