Background:

The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.

Applications:

ELISA, WB

Name of antibody:

FLAG Tag

Immunogen:

Synthetic peptide: DYKDDDDK conjugated to KLH

Full name:

FLAG Tag

Synonyms:

DDDDK epitope tag; DYKDDDDK epitope tag

ELISA Recommended dilution:

500-1000

WB Predicted band size:

50 kDa

WB Positive control:

Fusion proteins containing the Flag Tag

WB Recommended dilution:

10000-50000